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bodipy cholesterol  (MedChemExpress)


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    Structured Review

    MedChemExpress bodipy cholesterol
    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using <t>the</t> <t>BODIPY-cholesterol</t> assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
    Bodipy Cholesterol, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bodipy+cholesterol/BODIPY-Cholesterol/pmc13461153-215-6-9
    Average 95 stars, based on 33 article reviews
    bodipy cholesterol - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "CRISPR screening identifies DTX4 governing alveolar macrophage cholesterol efflux in pulmonary alveolar proteinosis"

    Article Title: CRISPR screening identifies DTX4 governing alveolar macrophage cholesterol efflux in pulmonary alveolar proteinosis

    Journal: JCI Insight

    doi: 10.1172/jci.insight.201129

    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using the BODIPY-cholesterol assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
    Figure Legend Snippet: ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using the BODIPY-cholesterol assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Techniques Used: Western Blot, Derivative Assay, Control, Cholesterol Assay, Staining, Flow Cytometry, Fluorescence

    Related Articles

    Labeling:

    Article Title: CDK13 phosphorylates KDM2A to activate a NUP93–STAT3–HMGCR cascade that promotes PDAC metastasis via cholesterol biosynthesis
    Article Snippet: .. Cell suspensions were labeled with BODIPY Cholesterol 1 μM) (HY-125,746, MCE, China), mixed thoroughly, and incubated t 37 °C for 15 minutes. ..

    Article Title: CRISPR screening identifies DTX4 governing alveolar macrophage cholesterol efflux in pulmonary alveolar proteinosis
    Article Snippet: .. In brief, cells were labeled with BODIPY-cholesterol (catalog HY-125746, MedChemExpress) by incubation in labeling medium (DMEM) containing 1× BODIPY-cholesterol and 2 μg/mL Sandoz (catalog S9318, Sigma-Aldrich), an acetylcholinesterase inhibitor, for 1 hour. .. After the labeling step, cells were washed and equilibrated in DMEM supplemented with 0.2% bovine serum albumin, 2 μg/mL Sandoz, and 0.3 mM cyclic adenosine 3′,5′-monophosphate (cAMP; catalog C8988, Sigma-Aldrich) for 16 hours.

    Article Title: Cholesterol Laundry of Cell Membrane and Fatty Liver by Detergent Liposomes to Improve Anti‐Cancer Drug Responsiveness of Patient Liver Tissues
    Article Snippet: .. The surface marker CD133 and membrane cholesterol of the nanovesicles were labeled with a red fluorescence conjugate (ab226355, Abcam, Cambridge, UK) and BODIPY‐cholesterol (HY‐125746, MedChemExpress, Monmouth Junction, NJ, USA), respectively, following the suppliers’ protocols. .. The endosome nanovesicles were incubated with liposomes for 2 h at room temperature, followed by flow cytometric analysis with FlowJo.

    Incubation:

    Article Title: CDK13 phosphorylates KDM2A to activate a NUP93–STAT3–HMGCR cascade that promotes PDAC metastasis via cholesterol biosynthesis
    Article Snippet: .. Cell suspensions were labeled with BODIPY Cholesterol 1 μM) (HY-125,746, MCE, China), mixed thoroughly, and incubated t 37 °C for 15 minutes. ..

    Article Title: CRISPR screening identifies DTX4 governing alveolar macrophage cholesterol efflux in pulmonary alveolar proteinosis
    Article Snippet: .. In brief, cells were labeled with BODIPY-cholesterol (catalog HY-125746, MedChemExpress) by incubation in labeling medium (DMEM) containing 1× BODIPY-cholesterol and 2 μg/mL Sandoz (catalog S9318, Sigma-Aldrich), an acetylcholinesterase inhibitor, for 1 hour. .. After the labeling step, cells were washed and equilibrated in DMEM supplemented with 0.2% bovine serum albumin, 2 μg/mL Sandoz, and 0.3 mM cyclic adenosine 3′,5′-monophosphate (cAMP; catalog C8988, Sigma-Aldrich) for 16 hours.

    Article Title: LEO1 loss promotes ER stress-adapted migration and cholesterol dependency in colorectal cancer
    Article Snippet: .. To assess cholesterol utilization, cells were incubated with BODIPY-cholesterol (MCE, HY-125746) (10 μM) for 12 hours. .. After incubation, cells were washed with PBS to remove excess probe and then replaced with either fresh complete medium or complete medium supplemented with orlistat (Merck, O4139) (20 μM).

    Marker:

    Article Title: Cholesterol Laundry of Cell Membrane and Fatty Liver by Detergent Liposomes to Improve Anti‐Cancer Drug Responsiveness of Patient Liver Tissues
    Article Snippet: .. The surface marker CD133 and membrane cholesterol of the nanovesicles were labeled with a red fluorescence conjugate (ab226355, Abcam, Cambridge, UK) and BODIPY‐cholesterol (HY‐125746, MedChemExpress, Monmouth Junction, NJ, USA), respectively, following the suppliers’ protocols. .. The endosome nanovesicles were incubated with liposomes for 2 h at room temperature, followed by flow cytometric analysis with FlowJo.

    Membrane:

    Article Title: Cholesterol Laundry of Cell Membrane and Fatty Liver by Detergent Liposomes to Improve Anti‐Cancer Drug Responsiveness of Patient Liver Tissues
    Article Snippet: .. The surface marker CD133 and membrane cholesterol of the nanovesicles were labeled with a red fluorescence conjugate (ab226355, Abcam, Cambridge, UK) and BODIPY‐cholesterol (HY‐125746, MedChemExpress, Monmouth Junction, NJ, USA), respectively, following the suppliers’ protocols. .. The endosome nanovesicles were incubated with liposomes for 2 h at room temperature, followed by flow cytometric analysis with FlowJo.

    Fluorescence:

    Article Title: Cholesterol Laundry of Cell Membrane and Fatty Liver by Detergent Liposomes to Improve Anti‐Cancer Drug Responsiveness of Patient Liver Tissues
    Article Snippet: .. The surface marker CD133 and membrane cholesterol of the nanovesicles were labeled with a red fluorescence conjugate (ab226355, Abcam, Cambridge, UK) and BODIPY‐cholesterol (HY‐125746, MedChemExpress, Monmouth Junction, NJ, USA), respectively, following the suppliers’ protocols. .. The endosome nanovesicles were incubated with liposomes for 2 h at room temperature, followed by flow cytometric analysis with FlowJo.



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    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using <t>the</t> <t>BODIPY-cholesterol</t> assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using <t>the</t> <t>BODIPY-cholesterol</t> assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using <t>the</t> <t>BODIPY-cholesterol</t> assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    Image Search Results


    ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using the BODIPY-cholesterol assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: JCI Insight

    Article Title: CRISPR screening identifies DTX4 governing alveolar macrophage cholesterol efflux in pulmonary alveolar proteinosis

    doi: 10.1172/jci.insight.201129

    Figure Lengend Snippet: ( A ) Western blot analysis of indicated proteins in differently treated THP-1–derived macrophages. GAPDH was used as a loading control. ( B ) Quantification of cholesterol efflux using the BODIPY-cholesterol assay ( n = 3 biological replicates). ( C and D ) Representative images ( C ) and quantification ( D ) of Oil Red O staining showing intracellular lipid droplet accumulation under different treatment conditions ( n = 3 biological replicates). Scale bars: 20 μm. ( E – G ) Flow cytometry analysis showing BODIPY fluorescence in THP-1–derived macrophages across treatment groups, including density plots ( E ), ridgeline plot ( F ), and quantification of BODIPY hi cell proportions ( G ) ( n = 3 biological replicates). ( H ) Confocal images of THP-1–derived macrophages stained with BODIPY 493/503 (green) to visualize neutral lipids ( n = 3 biological replicates); nuclei were stained with Hoechst 33342 (blue). Scale bars: 5 μm. ( I – K ) Quantification of intracellular free cholesterol ( I ), cholesteryl esters ( J ), and total cholesterol ( K ) in THP-1–derived macrophages under different treatments ( n = 3 biological replicates). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s post hoc test for multiple comparisons (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: In brief, cells were labeled with BODIPY-cholesterol (catalog HY-125746, MedChemExpress) by incubation in labeling medium (DMEM) containing 1× BODIPY-cholesterol and 2 μg/mL Sandoz (catalog S9318, Sigma-Aldrich), an acetylcholinesterase inhibitor, for 1 hour.

    Techniques: Western Blot, Derivative Assay, Control, Cholesterol Assay, Staining, Flow Cytometry, Fluorescence